[Histonet] need help about frozen bladder sections

From:"izzet oguz"

we took  bladder sections from rats about four months ago..first we froze
the tissue in liquid nitrogen. Then left them at -20 C deep freeze for 4
months..after that we could not cut the tissues because they became so
fragile..Any suggestions about this problem will be greatly appreciated..
Thanks..
›zzet




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