[Histonet] histogel

From:"Patsy Ruegg"

Has anyone ever tried using something like histogel to orient tissue for 
frozen sectioning?  I am wondering about the cutting and freezing quality of 
samples held in histogel til firm and then quick frozen in oct?  I need to 
get cross sections of mouse bile ducts.  I do this very successfully using 
histogel and paraffin processing, but I need to get frozen sections of the 
same for IHC T subset studies.
Please advise.
Best regards,
Patsy



Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
P-720-859-4060
F-720-859-4110
HP-303-644-4538
HF-303-644-3377
hm email pruegg@msn.com
wk email pruegg@colobio.com

This email is confidential and intended solely for the use of the Person(s) 
('the intended recipient') to whom it was addressed. Any views or opinions 
presented are solely those of the author. It may contain information that is 
privileged & confidential within the meaning of applicable law. Accordingly 
any dissemination, distribution, copying, or other use of this message, or 
any of its contents, by any person other than the intended recipient may 
constitute a breach of civil or criminal law and is strictly prohibited. If 
you are NOT the intended recipient please contact the sender and dispose of 
this e-mail as soon as possible.

_________________________________________________________________
Get McAfee virus scanning and cleaning of incoming attachments.  Get Hotmail 
Extra Storage!   http://join.msn.com/?PAGE=features/es


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


<< Previous Message | Next Message >>