[Histonet] histogel

From:"Patsy Ruegg"

Has anyone ever tried using something like histogel to orient tissue for 
frozen sectioning?  I am wondering about the cutting and freezing quality of 
samples held in histogel til firm and then quick frozen in oct?  I need to 
get cross sections of mouse bile ducts.  I do this very successfully using 
histogel and paraffin processing, but I need to get frozen sections of the 
same for IHC T subset studies.
Please advise.
Best regards,

Patsy Ruegg, HT(ASCP)QIHC
IHCtech, LLC
Fitzsimmons BioScience Park
12635 Montview Blvd. Suite 216
Aurora, CO 80010
hm email pruegg@msn.com
wk email pruegg@colobio.com

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