[Histonet] Rhabdomyosarcomas

From:"David Muskett"

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Dear Histonetters

One of my colleagues is currently trying to develop ISH within our lab to
help distinguish subtypes of rhabdomyosarcomas using probes to the PAX 3 and
PAX 7 genes. Our supply of probes has not materialised, as I would have
hoped.

My question is therefore in 3 parts

1.                 Does anyone have any experience of using probes for Pax3
and Pax7 genes?
2.                 Does anyone have a gene sequence for the probes?
3.                 Does anyone know of a source where I can get the probes
made, which is hopefully CE marked and compliant with the new European In
vitro Diagnostics Directive.

Any help or suggestions would be appreciated

David Muskett
Liverpool, UK

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Dear = Histonetters

 

One of my = colleagues is currently trying to develop ISH within our lab to help distinguish = subtypes of rhabdomyosarcomas using probes to the PAX 3 and PAX 7 genes. Our supply = of probes has not materialised, as I would have = hoped.

 

My question is = therefore in 3 parts

 

1.           = ;      Does anyone have any experience of using probes for Pax3 = and Pax7 genes?

2.           = ;      Does anyone have a gene sequence for the = probes?

3.           = ;      Does anyone know of a source where I can get the probes = made, which is hopefully CE marked and compliant with the new European In vitro = Diagnostics Directive.

 

Any help or = suggestions would be appreciated

 

David = Muskett

Liverpool, = UK

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