[Histonet] Re: RE: mast cells
I just want to let you know this!
Thanks for the info you had send me
----- Original Message -----
From: "C.M. van der Loos"
Date: Friday, October 3, 2003 10:56 am
Subject: RE: mast cells
> Hi Noreen,
> Apart from the histology solutions there is a very good IHC
> staining for mast cells too. We have been successful using a directly
> alk. phosphatase labeled anti-tryptase antibody from Chemicon
> Tryptase is an enzyme present in mast cells only. Beautiful staining
> on both cryostat (1:500, overnight 4C) or FFPE sections (HIER with
> citrate6.0, 1:500 overnight 4C). For alk. phosphatase chromogen
> use either New Fuchsin (Dako K0596), Permanent Red (Dako K0695) or
> Vector Red (SK-5100).
> Chris van der Loos
> Dept. of Cardiovascular Pathology
> Academical Medical Center
> Amsterdam - The Netherlands
> ----- Original Message -----
> From "Noreen Gilman"
> Date Wed, 01 Oct 2003 15:00:20 -0400
> Subject [Histonet] mast cells
> Can anyone recommend a stain for mast cells? The one we do just
> seem to be working as well as we'd like it to.
> Noreen Gilman, BS, HT (ASCP) QIHC
> Histopathology Supervisor
> Broward General Medical Center
> Ft. Lauderdale, FL 33316
Histonet mailing list
<< Previous Message | Next Message >>