[Histonet] RE: mast cells
Apart from the histology solutions there is a very good IHC staining
for mast cells too. We have been successful using a directly alk.
phosphatase labeled anti-tryptase antibody from Chemicon (MAB1222A).
Tryptase is an enzyme present in mast cells only. Beautiful staining on
both cryostat (1:500, overnight 4C) or FFPE sections (HIER with
citrate6.0, 1:500 overnight 4C). For alk. phosphatase chromogen use
either New Fuchsin (Dako K0596), Permanent Red (Dako K0695) or Vector
Chris van der Loos
Dept. of Cardiovascular Pathology
Academical Medical Center
Amsterdam - The Netherlands
----- Original Message -----
From "Noreen Gilman"
Date Wed, 01 Oct 2003 15:00:20 -0400
Subject [Histonet] mast cells
Can anyone recommend a stain for mast cells? The one we do just doesn't
seem to be working as well as we'd like it to.
Noreen Gilman, BS, HT (ASCP) QIHC
Broward General Medical Center
Ft. Lauderdale, FL 33316
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