[Histonet] Help; looking for a stain receipe for TTC
|From:||Bernard Ian R SSgt 59 CRES/MSROP |
Hello folk or fellow
histonetters(in particular in research),
I'm in the
process of replicating the histochemical staining method in a protocol
article titled " LV-Powered Coronary Sinus Retroperfusion Reduces infarct
Size in Acutely Ischemic Pigs."
The made reagents
for staining the heart tissue calls for a pre staining is a Phthalol-blue, which
we will be substituting with Evans Blue. I need a concentration
and receipe to make this up? The article does not
provide this. The article states that the " ascending aorta
was occluded and phthalo-blue(Evans Blue) was injected into the left
article states: " The left Ventricle was sliced, parallel to the
atriopventricular groove, into several 5-10 mm slices and incubated for 30 mins
at 37 deg C in TTC or Triphenyltetrazolium Chloride. There are 2
concentrations by Sigma, which one is to be used here, a 95% or 99.5 %?
Need receipe to make this solution?
Trisma HCL and Trisma Base? I need catalog #'s and
goes on to state for results:That "the Mycardial tissue containing
dehydrogenase enzymes is stained brick red by TTC, but areas of necrotic tissue
depleted of these enzymes are not stained and appear as white or pale
<< Previous Message | Next Message >>