[Histonet] DAKO ER and PR antibodies


Hi out there in Histonet land:

I am having a problem working up dilutions for DAKO's ER (M7047) and PR 
(M3569) antibodies.
I am using the Envision+ Detection system

Suggestions from DAKO were:

ER  1:100 to 1:150 with Envision+ and a 20 minute target retrieval
PR  1:200 to 1:400 with Envision+ and a 20 minute target retrieval.

I've gone less and more and still cannot get any where near the staining on 
these cases that went out to a IHC Laboratory than I am using as a 

Any suggestions would certainly be appreciated.

Thanks so much,
Theresa Rohr, Section Head-Histology
Nyack Hospital, New York

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