The immunohistochemical detection of cyclin D1 in formalin-fixed,
paraffin embedded tissue has been problematic.  Over the years, I have
tried to help many labs detect cyclin D1 by supplying our procedure, as
well as positive control tissue.  We recently evaluated a new rabbit
monoclonal antibody (clone SP4) from Lab Vision Corporation (Fremont,
CA) that has proved very impressive in my laboratory (when compared to
clone DCS-6).  If you are having trouble detecting this protein, I
recommend that you try this antibody.

Richard Cartun
Director, Immunopathology
Hartford Hospital
Hartford, CT  06102

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