Fwd: Re: [Histonet] MMA sections
Fwd: Re: [Histonet] MMA
This works very well, you need to de-resin in Xylene at 37 grees.
Two changes for 15 minutes each. Then go on with normal wax staining
process, i.e dehydrate, block primary etc, etc.
You can find papers written by Neil Hand on this subject. It is
tried and tested with great results.
Cardio Vascular Research
University of Cape Town
Date: Thu, 9 Oct 2003 08:16:20 +0200
From: Melanie Black <email@example.com>
Subject: Re: [Histonet] MMA sections
i need to do ummunostaining on MMA sections of 5 Um deplasticised in
xylene, i tried one time with a protocol used for paraffin section,
but it doesn't work, i deplasiticised MMA in aceton 2 X 10 minutes at
Please, do you have a protocol especially for MMA sections, or special
embedding in MMA for immunostaning, or any advice for
thank you very much in advance
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