Fwd: Re: [Histonet] MMA sections

From:Melanie Black

Fwd: Re: [Histonet] MMA sections
Hi Marseille

This works very well, you need to de-resin in Xylene at 37 grees. Two changes for 15 minutes each. Then go on with normal wax staining process, i.e dehydrate, block primary etc, etc.

You can find papers written by Neil Hand on this subject. It is tried and tested with great results.

Good Luck
Melanie Black
Cardio Vascular Research
University of Cape Town
South Africa.



Date: Thu, 9 Oct 2003 08:16:20 +0200
To: Myri37@aol.com
From: Melanie Black <ctsblack@capeheart.uct.ac.za>
Subject: Re: [Histonet] MMA sections
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Hello everyone
i need to do ummunostaining on MMA sections of 5 Um deplasticised in xylene, i tried one time with a protocol used for paraffin section, but it doesn't work, i deplasiticised MMA in aceton 2 X 10 minutes at 56 C
Please, do you have a protocol especially for MMA sections, or special embedding in MMA for immunostaning, or any advice for deplasticise..
thank you very much in advance
Myriam Baali

Natural Implant
Marseille


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