Fwd: Re: [Histonet] MMA sections
Fwd: Re: [Histonet] MMA
sections
Hi Marseille
This works very well, you need to de-resin in Xylene at 37 grees.
Two changes for 15 minutes each. Then go on with normal wax staining
process, i.e dehydrate, block primary etc, etc.
You can find papers written by Neil Hand on this subject. It is
tried and tested with great results.
Good Luck
Melanie Black
Cardio Vascular Research
University of Cape Town
South Africa.
Date: Thu, 9 Oct 2003 08:16:20 +0200
To: Myri37@aol.com
From: Melanie Black <ctsblack@capeheart.uct.ac.za>
Subject: Re: [Histonet] MMA sections
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Hello
everyone
i need to do ummunostaining on MMA sections of 5 Um deplasticised in
xylene, i tried one time with a protocol used for paraffin section,
but it doesn't work, i deplasiticised MMA in aceton 2 X 10 minutes at
56 °C
Please, do you have a protocol especially for MMA sections, or special
embedding in MMA for immunostaning, or any advice for
deplasticise..
thank you very much in advance
Myriam Baali
Natural Implant
Marseille
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