staining of free floating sections

From:Melissa Jans

I have some 100, 200 and 400 micron thick lung sections that a pathologist would like to have stained.  These sections are loose (not adhered to a slide) and they do not have paraffin in them (don't ask me how they did this but they did).  He would like me to do an H&E, Alcian Blue/PAS, Trichrome, and and Elastichrome.  Does anyone have experience doing this?  What is the best way to transfer your loose section from reagent to reagent?  Do I need to dilute my solutions and/or change my staining times? 

Any help would be appreciated, this is something totally new to me.

Thanks!!

Melissa Jans

Lead Scientist, Histology Lab

University of Iowa Healthcare



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