|From:||Melissa Jans |
I have some 100, 200 and 400 micron thick lung sections that a pathologist would like to have stained. These sections are loose (not adhered to a slide) and they do not have paraffin in them (don't ask me how they did this but they did). He would like me to do an H&E, Alcian Blue/PAS, Trichrome, and and Elastichrome. Does anyone have experience doing this? What is the best way to transfer your loose section from reagent to reagent? Do I need to dilute my solutions and/or change my staining times?
Any help would be appreciated, this is something totally new to me.
Lead Scientist, Histology Lab
University of Iowa Healthcare