silver stain and flurojade staining

From:jay cheung

Hi, everyone:
I am currently running silver staining and fluoro-jade staining for detecting neuronal degeneration in rat brain fixed and fresh frozen sections.  I never ran these procedures before and the results I got from the first trial showed many non specific staining not limited to degenerating neurons.  Also, it seems like the PFA fixed tissue got more silver stain than the fresh post-fixed tissue.  Can someone tell me the mechanism of action of these staining procedures and what are the tricks about these procedures.  I did some research myself and so far have no luck finding out the answers for these questions.  I used FD silver stain kit to do the silver stain.  Thank you so much for your help!

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