paraffin temps for immunos

From:Steven Slap

Hi Russ

Add into the mix of thoughts that you've received that all microwave tissue
processing requires that the paraffin be heated above the boiling point of
the intermedium, usually isopropanol, (in which case it is 72 degrees C).
Far from compromising the integrity of immunos, a test with mirror blocks
done at the Univ. of Louisville by Dr. Jeff Sosnowski (with help from Sheron
Lear) showed better preservation of antigenicity for both IHC and FISH with
microwave-processed tissue than with conventionally processed tissue taken
from the same block.

best regards,
Steven Slap

Marketing Manager/Microwave Product Specialist
Hacker Instruments & Industries, Inc.

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