HI Melissa,
I don't have a lot of experience with this, but what I did do for 
someone else one time, was to cut 50-80 um frozen sections and 
place them directly in a 24-well plate (each section to its own well).
The 24-well plate was kept in the cryostat and the fixative (I forget 
now, which one they were using) was already dispensed to all the 
wells. All subsequent staing took place in the well to minimize 
tissue handling (ie the reagents were changed rather than moving 
the tissue sections). I wasn't present to see the sections get 
mounted on the slide, but I understand it was a delicate process!
Good luck.
 

Date sent:      	Tue, 29 Oct 2002 11:39:17 -0800 (PST)
From:           	Melissa Jans 
Subject:        	staining of free floating sections
To:             	histonet@pathology.swmed.edu

> 
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> I have some 100, 200 and 400 micron thick lung sections that a pathologist would like to have stained.  These sections are loose (not adhered to a slide) and they do not have paraffin in them (don't ask me how they did this but they did).  He would like me to do an H&E, Alcian Blue/PAS, 
Trichrome, and and Elastichrome.  Does anyone have experience doing this?  What is the best way to transfer your loose section from reagent to reagent?  Do I need to dilute my solutions and/or change my staining times?  
>  
> Any help would be appreciated, this is something totally new to me.
>  
> Thanks!!
>  
> Melissa Jans
>  
> Lead Scientist, Histology Lab
>  
> University of Iowa Healthcare
>  
> 
> 
> ---------------------------------
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> 
I have some 100, 200 and 400 micron thick lung sections that a pathologist would like to have 
stained.  These sections are loose (not adhered to a slide) and they do not have paraffin in t
hem (don't ask me how they did this but they did).  He would like me to do an H&E, Alcian 
Blue/PAS, Trichrome, and and Elastichrome.  Does anyone have experience doing this?  What
 is the best way to transfer your loose section from reagent to reagent?  Do I need to dilute 
my solutions and/or change my staining times?  
 
> 
Any help would be appreciated, this is something totally new to me.
 
> 
Thanks!!
 
> 
Melissa Jans
 
> 
Lead Scientist, Histology Lab
 
> 
University of Iowa Healthcare
 

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obs daily now
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