Re: skull decalcification

From:Patsy Ruegg

are you sure y ou need to decalcify

Matt McElwee wrote:

> Hi everyone.  Does anyone have a good way to decalcify mouse skulls that
> doesn't shrink or otherwise alter the brain?  We want to section mouse
> heads from neonates through 2 weeks of age.  I know that we'll have to
> change the time that the brain is in the decalcifying solution,
> depending on the age of the mouse, but if anyone can recommend a good
> decalcifying solution or give any other advice, I'd really appreciate
> it.
> Thank you.
> Matt
> Matt McElwee
> Research Specialist
> Department of Surgery
> K4/617 Clinical Science Center
> 600 Highland Avenue
> Madison, WI 53792
> (608) 263-7648

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