Re: eNOS staining

From:Albert Grobe

We have done eNOS (eNOS/NOS Type III) on sheep tissues using a biotin/streptavidin detection from DAKO (LSAB2) with a DAB development.  The primary is from BD Biosciences.  They also carry Ab to Type I and II.  

Albert C. Grobe, MS
Tissue Engineering Lab
International Heart Institute
Missoula, MT

>>> Cheryl Crowder  10/17/02 08:28AM >>>
Hello - I have a doctor who wants to do eNOS I, II, III on paraffin 
embedded tissues.  Have any of you done this IHC and can it be done on 
paraffin sections?  From whom did you get you primary Abs?  He wants to 
get primaries from Santa Cruz but they have been no technical help.  Also 
what type of secondary did you use (type of kit)?  We will have mega-
amounts of tissue to stain and we don't have a lot of time to make 
mistakes trying things out.  I would really appreciate any help you can 
give.  Thank you.  Cheryl

Cheryl Crowder, BA, HTL(ASCP)
Chief Technologist
Department of Pathobiological Sciences
School of Veterinary Medicine
Louisiana State University
Baton Rouge, LA #160#70803

(225) 578-9784
FAX #160#(225) 578-9720

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