jim wrote:

> has anyone had experience with myelin stains on thick sections i.e. 30-50
> microns.

Yes, I have. The original luxol fast blue method works well
on freezing microtome sections that have been dried down onto 
slides. Neutral red is a nice counterstain and it also
intensifies the blue colour of the myelin.
-- 
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan@uwo.ca
   http://publish.uwo.ca/~jkiernan/