Re: Questions about staining solutions.

We change our stains weekly,basically we use slide volume rather than ph, but since the higher number of slides, the more carryover of alcohols or water from washes, then obviously ph changes,we change certain alcohols and xylenes daily, others we rotate all of course having an effect on the h&e quality.we also run a test h&e slide every morning, i suggest you may want to do this with your trichromes and can possibly determine maximum number of slides before quality gives out. just one more histotechs opinion.

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