> Sofian Johar wrote:
> I am looking at frozen sections of equatorial sections of mice
> left ventricles. I am currently staining for 1 hour but have
> widespread areas where the myocytes are stained red rather than
> yellow (bright field microscopy). I was wondering if the length
> of time of staining is different from paraffin sections from
> frozen sections (do you stain for less time?).

Bad fixation is probably the cause of your difficulties.
How were the hearts fixed before being frozen? Why are you
doing picrosirius red on frozen, rather than paraffin
sections?

-- 
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
   kiernan@uwo.ca
   http://publish.uwo.ca/~jkiernan/