Re: Lung sectioning and Imaing

From:Eman Namati

I would like to get serial sections of an entire mouse lung. 3 micron
thickness every 30 microns.

For the human lung i would like to embedd the lung in half inch slabs of
200x150mm, so i can section it using a polycut microtome. I will be imaging
these off the block of remaining tissue using a stereoscope placed above the
microtome on a xyz gantry which will increase the field of view by acquiring
multple shots and creating one high res image of the entire section.

I would like to know if accurate serial sections through out an entire mouse
lung using conventional methods is possible or not? and has anyone tried
embedding whole human or sheep lung?

Eman Namati
Project Manager, Lung Image Database Consortium
Roy J. and Lucille A. Carver College of Medicine
University of Iowa
200 Hawkins Dr., 378 MRF
Iowa City, IA 52242
Tel: (319) 384-8331
Mob: (319) 936-5487
Fax: (319) 353-6406

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