Dear John
After you use crystal violet, Gram Iodine  and counterstain, towards the end 
of the stain you have to use a 50% solution of xylene and acetone. Then take 
it into the xylene and coverslip. There is a step where Bouins and Formalin 
mixture is used.
I am trying to get rid of the xylene/acetone step. I don't have the procedure 
at home with me. Its just a stain that the lab uses where I work.
Thank you 
sandi miller HT
USAMRICD Research
Maryland