| From: | James L Burchette |
Rabbit anti-human FXIIIa Calbiochem catalog # 233498, diluted 1:1500 for 1 hour. I pretreat with Brigati's Stable Pepsin for 13 minutes at 37-40C. Biotin labeled secondary followed by HRP labeled streptavidin, 20 min each. Jim Burchette Duke University Medical Center Immunopathology Lab