Re: Experience with CD4 and CD8 on paraffin sections?

From:Patti Loykasek

Re: Experience with CD4 and CD8 on paraffin sections?
Here’s our protocol for CD4 on paraffin embedded. I have found it to be a ‘stubborn’ antibody. Most of the time it works fine, but there are some less than beautiful days. Hope this helps.

Antibody: CD004
Clone: 1F6
Vendor: Novocastra
Antigen Retrieval: Steam 40 minutes in EDTA, pH8
Titer/Incubation: 1:10 for 40 minutes at room temperature
Detection: DAKO Envision+, mouse for 30 minutes at room temperature
Chromogen: DAB

Patti Loykasek
Phenopath Loykasek
Seattle, WA




Hello everyone,
    I am shopping for antibodies against human CD4 and CD8 to be used on paraffin-embedded tissue. I found 4 clones in NovoCastra, two against CD4 (1F6 and 4B12) and two against CD8 (4B11 and 1A5). Does anyone have experience with these clones? Thanks in advance,
Emmanuel Maicas, MD in Moncton, Canada



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