Re: ? Crystal Mount artefact
Could these be starch granules washed off from powdered surgical gloves?
Bone Research Unit
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>From: Anissa Choi
>Subject: ? Crystal Mount artefact
>Date: Tue, 22 Oct 2002 00:22:48 -0700
>We run our immuno slides with AEC on Ventana immunostainer. After
>staining, we place them in 1% Dawn detergent for 5 min to remove the liquid
>coverslip, followed by thorough washing in water. Slides are then covered
>with Crystal Mount and dry in oven for 20 to 30 min.
>From time to time, we notice there are some tiny, black, refractile
>round granules on top of the tissue section. Sometimes, it is so bad
>that every part of the section is covered with this artefact granules,
>which I think may be related to the Crystal Mount. I am seeking help to
>solve this problem. Thanks in advance for your input.
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