Re: Chloroacetate Esterase

From:Patsy Ruegg

not much up on billing but this is a common enzyme histochemical stain usually
done for hematopathology cases
patsy

"DeLovino, Salvacion S." wrote:

> What's the billing code for this? It's just a coincidence that it has come
> up in our lab while I'm reading this. Thanks in advance.
>
> Salvacion
>
> > ----------
> > From:         Patsy Ruegg[SMTP:pruegg@colobio.com]
> > Reply To:     pruegg@colobio.com
> > Sent:         Wednesday, October 16, 2002 12:39 PM
> > To:   DeLovino, Salvacion S.
> > Cc:   Histonet (E-mail); 'Johnston, Kathy'
> > Subject:      Re: Chloroacetate Esterase
> >
> > when i had a b5 fixed sample to do TRAP on i just put up with the mercury
> > parcipitate, it usually did not pose an interpation problem.
> > patsy
> >
> > "DeLovino, Salvacion S." wrote:
> >
> > > In our old procedure, there was a comment section that says esterase is
> > > inhibited to varying degrees by mercury, acid solutions, heat, and
> > iodine.
> > >
> > > Salvacion Delovino
> > > > ----------
> > > > From:         Johnston, Kathy[SMTP:Kathy.Johnston@CLS.ab.ca]
> > > > Sent:         Friday, October 11, 2002 8:39 AM
> > > > To:   Histonet (E-mail)
> > > > Subject:      Chloroacetate Esterase
> > > >
> > > > We are using Sigma's Chloroacetate Esterase kit, and are doing one on
> > a B5
> > > > fixed bone marrow slide.  Our problem is thus:  Both myself and the
> > stains
> > > > tech recall in the recesses of our collective memories, that Na
> > > > thio/iodine
> > > > to remove the mercury pigment produces a false negative for this test.
> > > >
> > > > Is there anyone else out there that confirms or contradicts this??  We
> > are
> > > > reluctant to proceed and possibly ruin a slide, as well, we do not
> > want to
> > > > stain it, and leave a bunch of mercury which may impair diagnosis.
> > > >
> > > > Your help is greatly appreciated!
> > > >
> > > > Kathy Johnston
> > > > Tech II - Special Stains
> > > > Anatomic Pathology - Foothills Medical Center
> > > > Calgary Laboratory Services
> > > > Ph - 403-944-4760
> > > > Fax - 403-270-4093
> > > > kathy.johnston@cls.ab.ca
> > > >
> > > >
> >




<< Previous Message | Next Message >>