RE: staining of free floating sections

From:"Charles W. Scouten, Ph.D."

Vibratome company has a new tray setup called the Histodipper for moving 24 wells of free floating sections from reagent to reagent.  It is designed for IHC to minimize needed antibody reagents.   See it at the following:
Be sure to go to the tan box to the right of the picture and click more images for detailed pictures.
If the link doesn't work, go to, click marketplace, scroll down to histology, and select under that heading the staining apparatus line.  Select the histodipper.
Charles W.  Scouten, Ph.D. 
5918 Evergreen Blvd. 
St. Louis, MO 63134 
Ph: 314 522 0300  
FAX  314 522 0377 
-----Original Message-----
From: Melissa Jans [] 
Sent: Tuesday, October 29, 2002 1:39 PM
Subject: staining of free floating sections
I have some 100, 200 and 400 micron thick lung sections that a pathologist would like to have stained.  These sections are loose (not adhered to a slide) and they do not have paraffin in them (don't ask me how they did this but they did).  He would like me to do an H&E, Alcian Blue/PAS, Trichrome, and and Elastichrome.  Does anyone have experience doing this?  What is the best way to transfer your loose section from reagent to reagent?  Do I need to dilute my solutions and/or change my staining times?  
Any help would be appreciated, this is something totally new to me.
Melissa Jans
Lead Scientist, Histology Lab
University of Iowa Healthcare

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