I'm having the same problem here.  I agree with the temp but still working
on it. Hope we get some response.


Rudy Salcedo
Research Assistant II
SCEH&M
Research Histopathology Core Lab
UTMB-Galveston

		-----Original Message-----
		From:	Andrea Grantham [mailto:algranth@u.arizona.edu]
		Sent:	Wednesday, October 16, 2002 6:53 PM
		To:	histonet@pathology.swmed.edu
		Subject:	frozen frog oocytes

		I'm trying to section Xenopus oocytes frozen in OCT with
poor results. Lots 
		of cracking and shattering. I'm figuring that temp plays a
roll here and 
		the little things are very "yolky".
		Is there anybody out in histonetland who may have done some
sectioning of 
		frog oocytes or something similar? I actually found a
Xenopus histo book 
		but no suggestions for cryosectioning.
		As is usually the case here, the labs bring the tissue
already frozen in 
		OCT. What can I suggest they do prior to or during freezing
the make the 
		sections cut better?
		Does anybody know an optimal temp for cutting frog oocytes?
		Thanks for any help.

		Andi Grantham
	
.....................................................................
		: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology &
Anatomy     :
		: Sr. Research Specialist       University of Arizona
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		: (office:  AHSC 4212)          P.O. Box 245044
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USA       :
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algranth@u.arizona.edu)       :
	
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