RE: Questions about staining solutions.

From:"Johnston, Kathy"

We change our solutions for M. Trichromes every two weeks, and they are used
daily with slide counts ranging from 5 to 50 or more per day.


-----Original Message-----
From: Coleman, Jeffrey []
Sent: Thursday, October 17, 2002 1:52 PM
To: HistoNet Server (E-mail)
Subject: Questions about staining solutions.

I'm trying to set up a schedule for changing the stains we use in our Mikrom
Robot Stainer. However, I'm having a problem figuring out how long some of
the stains are usable. 

For H&E stains, I've found some literature suggesting the hematoxylin can be
used for up to 6 months, some say 2 months, others say depending on the pH.
Does anyone know what the pH for a good hematoxlin stain should be? How
often should the hematoxylin be changed?

We also use the Robot Stainer for Masson TriChrome stains. We've got a
changing schedule for all the solutions except Biebrich Scarlet/Acid Fuchsin
solution and Aniline Blue Stain solution. Any suggestions as to how often
these solutions should be changed?

Thanks in advance!

Jeff Coleman
Pharmacology, Canji
Schering-Plough Co. 

 This message and any attachments is solely for the intended recipient. If
you are not the intended recipient, disclosure, copying, use, or
distribution of the information included in this message is prohibited --
please immediately and permanently delete this message.

<< Previous Message | Next Message >>