Joyce,
I use osteopontin from the U of Iowa Hybridoma Bank instead of SC, with
pepsin digestion (premade pepsin from Research Genetics) with good results
on ffpe formic acid decalcified bone.
Patsy Ruegg

Original Message:
-----------------
From: joyce judge joycejudge259@hotmail.com
Date: Mon, 28 Oct 2002 13:31:37 -0500
To: histonet@pathology.swmed.edu
Subject: Osteopontin



I have checked the archives and have not found any info to guide me.  I am 
currently trying to work out the conditions for Santa Cruz 
Osteopontin(sc-10591) in paraffin sections of bone (or any other paraffin 
tissues).  I have tried Antigen Retrieval with Citra and EDTA, Prot K 
digestion and no pretreatment, at dilutions of 1:50 and 1:100 and have 
gotten no staining at all.  The company has no data on paraffin or Frozen 
section immunos. Any help is appreciated.

Joyce Judge
Ardais Corp
128 Spring Street
Lexington, MA 02421





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