RE: Hypotonic HEPES? Cell isolation?
|From:||"Prodhan, Parthak,M.D." |
I was wondering if there is anyone out there who could tell me what a hypotonic
Secondly does anyone have experience with cell type isolation (type II
epithelial cells and myofibroblasts from mouse/ rat lungs using percoll or some
other density gradient system. Would greatly appreciate inputs.
MassGeneral Hospital for Children
> From: Lauren Robinson
> Sent: Tuesday, October 29, 2002 5:26 PM
> To: email@example.com
> Subject: Standardization Protein for Nuclear Extracts
> Hello All,
> I was wondering if there was anyone out there who is doing Western Blots on
> nuclear extracts, and if so, what antibody they are using to standardize
> loading amounts? Thank you all for your help,
> Lauren Robinson
> Research Technician
> Department of Neurology
> The Children's Hospital of Philadelphia
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