RE: Fixation and Processing

From:Tony Henwood

You are quite lucky in that the tissues should be very well fixed having been in fixative over the weekend. I would leave the tissues in 70% ethanol before processing. They do not need refixing. Also short "deprocessing" (!!) times are advisable. You mentioned 10 min processing, I would use 10 minutes in xylene and ethanol before 70% ethanol

Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager
The Children's Hospital at  Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: (02) 9845 3306
Fax: (02) 9845 3318

-----Original Message-----
From: Peterson Lab []
Sent: Wednesday, 23 October 2002 1:06
Subject: Fixation and Processing

First off, I would like to thank all who attended and presented at the NSH Convention in Long Beach.  It was my first time and I had a wonderful time.  I came back really eager to start new projects and ideas.  I have only been a registered histotech for a year now and a full time histotech for 5 months and I still have many questions.
My question today is as follows.  Our tissue processor was set for weekend run last night and when I came in at 4:30, the processor hadn't started yet.  I ran a short run on the processor consisting of 10 minutes in each step in order to process the tissue for the day.  Many of the bigger pieces of tissue didn't cut well at all and were mushy.   I plan to reprocess these tissues and was wondering how long to leave them in formalin when our processor run for today doesn't start until 6:00 tonight.  My other question is that one of the tissues was a breast tissue that was positive and needs immunostains.  Do I put these tissues in 70% ethanol all day and then put them on the processor?
I know that this is a long question, but I thought it necessary to explain the situation in detail.  Thanks so much for your time!
Travis Troyer

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