I was wondering if there is anyone out there who has successfully done
Notch-1 staining on paraffin sections. If there is, would they be able to
tell me of the antibody, fixation/embedding protocol, and subsequent
treatments, if any. I have successfully gotten Notch antibodies to work on
frozen sections, but as we all know, (grin) paraffin sections are another
ball of wax entirely!
Department of Neurology
The Children's Hospital of Philadelphia
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