Notch-1 Staining

From:Lauren Robinson

Hello All,
I was wondering if there is anyone out there who has successfully done 
Notch-1 staining on paraffin sections. If there is, would they be able to 
tell me of the antibody, fixation/embedding protocol, and subsequent 
treatments, if any. I have successfully gotten Notch antibodies to work on 
frozen sections, but as we all know, (grin) paraffin sections are another 
ball of wax entirely!
                   Thank you,
                     Lauren Robinson
Department of Neurology
The Children's Hospital of Philadelphia

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