Cytotech Opportunity in West Texas
Good Afternoon,
My name is Josh Hatcher, I'm with Management Recruiters in Cordova, TN.
I've got an opportunity for a Cytotech in West Texas south of Lubbock.
You'll join a team of three Cytotechs and four to five Pathologists in this
outstanding clinical lab.
If you would be interested in this opportunity, or know someone who would
be, please email me at JHatcher@mricordova.com.
Thanks,
Josh Hatcher
MRI Cordova, Inc.
----- Original Message -----
From: "HistoNet Server"
To: "HistoNet Server"
Sent: Sunday, October 27, 2002 11:00 PM
Subject: Daily Digest
>
> ----------------------------------------------------------------------
>
> Date: 27 Oct 2002 11:15:56 -0500
> From: Romy Thomas
> Subject: Detecting GFP in Autofluorescent Bone Samples
>
> Dear All,
> Here is the problem:
> Strong autofluorescence of bone masking any underlying GFP signals from
> cells.
> (We have tried the dual chroma filter of FITC & Texas red without much
> improvement)
> Here are the questions:
> 1) What is the best way to fix and section bone samples to detect GFP
> signals?
> 2) Any suggestions on the appropriate filter and source would be great
> 3) Any opinions on reporter gene signals that survive EDTA decal
procedures?
>
> Many thanks
> Romy Thomas
>
>
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 27 Oct 2002 14:16:02 -0500
> From: pruegg@colobio.com
> Subject: frozen sections revisited
>
> Histonetters,
> I know this subject has been done on HISTONET before, but can we open this
> up again. how do you process frozen sections to get the best slide
> adhesion?
> do you fix immediately in cold fix in the cryostat or airdry and then fix?
> what do you fix in, ie cold acetone,etc.? do you always try to stain
> slides soon after preparing? do you store FS for staining later? how are
> FS stored, ?wrapped individually in foil and plastic bags, etc.? do you
> store them unfixed or fixed? how are stored FS retrieved from the
freezer?
> Thanks for responding to this mini survey.
> Patsy Ruegg
>
> James,
> are you talking about cells in suspension or tissue sections lifting off
> silane coated slides?
> for keeping FS on plus slides, i pick them up and let them airdry well for
> at least 10 min. at RT. there are some different methods for what to do
> after drying to fix and stain right away or store frozen unfixed until
> ready to stain, but i think the initial drying at RT goes a long way to
> keep them on the slides. some people have a jar of cold fixative in the
> cryostat and fix immediately after sectioning in the cryostat and then air
> dry, but i have had trouble with preservation of morphology doing it this
> way. people have their favorite methods that work the best in their
hands.
> my WS partner said she had some antigenicity loss from storing dryed
> unfixed sections in the freezer, but i have always done it that way with
no
> apparent problems.
>
> Original Message:
> - -----------------
> From: james.zimmerman@pharma.novartis.com
> Date: Fri, 25 Oct 2002 11:52:45 -0400
> To: Histonet@pathology.swmed.edu
> Subject:
>
>
> Has anyone had any problems with lifting off cells for LCM that have been
> picked up on SuperFrost Plus Slides? How
>
> did you resolve the problem?
>
> Also does anyone have any suggestions for keeping fresh frozen cyrostat
> sections on any kind of slide.
>
>
>
> Thanks,
>
> JPZ
>
>
>
> - --------------------------------------------------------------------
> mail2web - Check your email from the web at
> http://mail2web.com/ .
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 27 Oct 2002 14:16:18 -0500
> From: pruegg@colobio.com
> Subject: VEGF
>
> You could use about any vascular tissue for a VEGF control, I like tonsil
> because it is not all over the place, just a few isolated areas. Finding
a
> positive control for VEGF is not a problem, my problem is finding a neg.
> tissue control, I use cartilage even though it is not easy to work with
and
> even cartilage is not completely avascular. Someone suggested toe nails
or
> hair but they are also very difficult to work with. Any of you anatomy
> guru's got any ideas for neg. tissue control for VEGF?
> Patsy
>
> Original Message:
> - -----------------
> From: Anne Undersander under017@tc.umn.edu
> Date: Fri, 25 Oct 2002 06:28:45 -0500
> To: histonet@pathology.swmed.edu
> Subject: VEGF response
>
>
> Thanks for responses on supplier for VEGF antibody. One more questions.
> What type of tissue would you use as a positive control?
>
> Thanks.
> Anne Undersander HT(ASCP)
> Vet. Med./Animal Science
> University of Minnesota
>
>
> - --------------------------------------------------------------------
> mail2web - Check your email from the web at
> http://mail2web.com/ .
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 27 Oct 2002 15:30:25 -0500
> From: Barbara Murray
> Subject: Books
>
>
> Greetings,
> I would like suggested books that I can purchase for a tech that we will
be
> training for Histology. Cytology books would be helpful too.
> Thank in advance for your replies and have a great day!
>
> Barbara A. Murray, HT. (ASCP)
> Pathology Dept.
> The Alaska Native Medical Center
> Anchorage, Alaska 99508
> (907) 729-1804
>
>
>
> ******************* NOTE *******************
> There may be important message content
> contained in the following MIME Information.
> ********************************************
>
>
> - ------------------ MIME Information follows ------------------
>
> This is a multi-part message in MIME format.
>
> - --Boundary_(ID_H/Wdvw6QTfe8xCjzc2lpjw)
> Content-type: text/plain; charset=iso-8859-1
> Content-transfer-encoding: 7BIT
>
> <<<<<< See above "Message Body" >>>>>>
>
> - --Boundary_(ID_H/Wdvw6QTfe8xCjzc2lpjw)
> Content-type: text/html; charset=iso-8859-1
> Content-transfer-encoding: 7BIT
>
>
>
>
>
>
>
>
> Greetings,
> I would like suggested books that I
can
>
> purchase for a tech that we will be training for Histology. Cytology
> books
> would be helpful too.
> Thank in advance for your replies and have
a
> great day!
>
> Barbara A. Murray, HT. (ASCP)
> Pathology Dept.
> The Alaska Native Medical
Center
> Anchorage, Alaska 99508
> (907) 729-1804
>
> - --Boundary_(ID_H/Wdvw6QTfe8xCjzc2lpjw)--
>
>
> ----------------------------------------------------------------------
>
> Date: 27 Oct 2002 17:16:09 -0500
> From: Jeff and Wanda Gray
> Subject: RE: The future of Histotechs
>
> Just an interesting side note here:
> I took some courses at a college in Texas (trying to get the same degree
> discussed below), and noticed the school had an "Allied Health Society"
for
> people who didn't want to be doctors or nurses. I stopped by the advisors
> office one afternoon, on a rare day off to see what she had about
histology
> as a career. This woman, a former registered nurse, had no idea what that
> was. I quickly informed her, and gave her the NSH website address, and
any
> other information I could think of, but I was flabbergasted! Offered to
> speak at a meeting, but ran into the old "all meetings are at noon on a
> weekday" problem.
> I do try to get to my kids' schools career days, and I tell everyone what
I
> do and why I love it. As are most of us, I am too busy to do much more.
> Wanda Shotsberger Gray
>
>
> -----Original Message-----
> From: Bartlett, Jeanine [mailto:jqb7@cdc.gov]
> Sent: Wednesday, October 23, 2002 2:00 PM
> To: 'PMarcum'; JOE NOCITO; Morken, Tim; histonet@pathology.swmed.edu
> Subject: RE: The future of Histotechs
>
> When I was a sophomore in college, way back in 1975/76, I was talking to
my
> advisor about where I wanted to go with my college career. I was
interested
> in biology but did not want to be a doctor or a nurse. She advised me to
> look into a variety of things, one of which was histology. I had never
> heard of it. I visited hospitals and looked into the various fields she
> suggested but the first time I walked into a histology lab and smelled the
> xylene I knew I was hooked. (Figuratively speaking, of course!) The only
> bad part was when I told her my decision she said, "Oh! That doesn't even
> require college, you can go straight into the 12 month program if you're
> accepted." (And I've been trying to go back and complete that degree ever
> since, but that's another story for another time!) I guess what I'd like
to
> know is this: Do high school and college advisors discuss the career of
> histology when talking to students about the laboratory sciences? Last
fall
> my son was taking an anatomy course in high school. When they came to the
> histology portion I asked to come and speak to the class. I came armed
with
> literature that I ordered from NSH about the field and distributed it and
> answered questions. Most of the students seemed very interested. I left
> the surplus literature with the counselors office. Now if we could just
get
> more people to help get the word out it might just be a start.
>
> Jeanine Bartlett, HT(ASCP)
> Centers for Disease Control
> Infectious Disease Pathology Activity
> 1600 Clifton Rd., N.E. MS-G32
> Atlanta, GA 30333
>
>
> - -----Original Message-----
> From: PMarcum [mailto:pmarcum@polysciences.com]
> Sent: Wednesday, October 23, 2002 12:17 PM
> To: JOE NOCITO; Morken, Tim; histonet@pathology.swmed.edu
> Subject: RE: The future of Histotechs
>
>
> It would be hard to saturate a market that is in the grips of the shortage
> we have. We (meaning us older histotechs) need to get out and talk to
more
> of the high school career day students and community colleges as well as
> push for better training and more schools. Unfortunately we have a
problem
> most are too busy to it.
> We keep waiting for NSH or ASCP to help and they are limited also. I run
> into people all of the time who have no idea what Histology is, so how
would
> they even know where to look to find out about a career.
> We need to get loud!!! Doesn't anybody remember how to organize a protest
> anymore? We need to reverse it and find a way to get attention for a
career
> and rewards in Histology. If we start to get better people and train them
> (along with how to ask for more money) we will get what we want. Pam
Marcum
> (Neil and Patsy had some very good points.)
>
> > -----Original Message-----
> > From: JOE NOCITO [mailto:JNOCITO@satx.rr.com]
> > Sent: Monday, October 21, 2002 7:31 PM
> > To: Morken, Tim; histonet@pathology.swmed.edu
> > Subject: Re: The future of Histotechs
> >
> >
> > I know some cities in Texas are setting up histology programs at the
> > community college level. Here in San Antonio, our third class just
> > graduated in August and we just started another class of 10 students.
> > When we started the program here in San Antonio, the question
> > that came
> > to mind first was "are we going to saturate the market?" Well, up to
this
> > point, no. After three classes, there are still shortages in San
> > Antonio and
> > I know a lot more in Houston, Dallas and El Paso.
> >
> > Joe Nocito, BS, HT (ASCP) QIHC
> > Histology Manager
> > Pathology Reference Lab
> > San Antonio, Texas
> > ----- Original Message -----
> > From: "Morken, Tim"
> > To:
> > Sent: Monday, October 21, 2002 11:30 AM
> > Subject: RE: The future of Histotechs
> >
> >
> > > The school issue is kind of moot since there are so few histo schools
> > around
> > > (I think 24 in all the US at last count). In meeting hundreds of
> > histotechs
> > > over the years, only a handfull went through a histo program. The vast
> > > majority are on-the-job trained. Granting that the ideal tech is
> > > specifically trained, I feel the real issue is that people are
> > unaware the
> > > field even exists. That is a failing of pathologists and lab
> > managers, in
> > my
> > > opinion, who have ignored their duty to get people interested in the
> > field.
> > > Are histotechs really supposed to feel a responsibility to go out and
> > > recruit their replacement, even in light of any feeling loyalty they
may
> > > feel to the profession?
> > >
> > > BTW, the Atlanta Journal Constitution has a sunday feature called Why
I
> > Love
> > > My Job. Beside the main story they put a side panel Called "hot
> > jobs". Two
> > > weeks ago they highlighted histotechnology, and did a good job of it.
> > >
> > > some are taking the bull by the horns and opening new schools.
> > There is a
> > > new one at Dalton College in Albany Georgia, and a new one
> > opened a couple
> > > years ago in Califorina (mt san antonio college).
> > unfortunately, one also
> > > closed in seattle, leaving the entire west coast with only one histo
> > school
> > > - still.
> > >
> > > Hmmm, maybe THAT is the new career path!
> > >
> > > Tim Morken
> > > Atlanta
> > >
> > > -----Original Message-----
> > > From: Bartlett, Jeanine
> > > Sent: Monday, October 21, 2002 11:56 AM
> > > To: 'Dawson, Glen'; Morken, Tim; histonet@pathology.swmed.edu
> > > Subject: RE: The future of Histotechs
> > >
> > >
> > > Another issue is that the graduates that do come into the field
> > usually do
> > > not then attend a school of histotechnology. So you have educated
> > > individuals being "trained on the job". And we all know that
> > learning as
> > > you go is not the same as a structured 12-24 month program with the
> > > concentration that you receive in an accredited program. But how many
> > > college graduates want to take on the additional training at the
salary
> > that
> > > is usually offered? So we have that "anybody walking in can be
> > trained to
> > > do this job" mentality. I know of individuals that have walked
> > into a lab
> > > with a degree but no histology laboratory experience at all and
> > are hired
> > at
> > > a higher salary than those without the degree but with formal
histology
> > > training. That does not help the perception of our chosen field.
> > >
> > > Jeanine Bartlett, HT(ASCP)
> > > Centers for Disease Control
> > > Infectious Disease Pathology Activity
> > > 1600 Clifton Rd., N.E. MS-G32
> > > Atlanta, GA 30333
> > >
> > >
> > >
> > >
> > > -----Original Message-----
> > > From: Dawson, Glen [mailto:GDawson@Milw.Dynacare.com]
> > > Sent: Monday, October 21, 2002 11:30 AM
> > > To: Morken, Tim; histonet@pathology.swmed.edu
> > > Subject: RE: The future of Histotechs
> > >
> > >
> > > I hope you are right Tim. There is a huge resource that histology
could
> > tap
> > > into; college graduates with a bachelor's degree in Biology since many
> > > finish school and cannot find a job that they are qualified for. The
> > > problem is that it is difficult approaching these graduates with a
> > > histotech's salary without apologizing for the low figure. These
folks
> > > would be great additions to the histology lab but, as of now,
> > the rewards
> > of
> > > histology aren't good enough to entice them in.
> > > I fear that the field is so low on the perceived "importance totem
pole"
> > > that the crisis will be MAJOR before lab management truly addresses
the
> > > problem. I have an interesting take on the histology situation from
one
> > > mavery@pcllab.com, a lab manager who's views on the field were so low,
I
> > > can't post them to this listserver for fear he may never receive a
> > Christmas
> > > card from any of us again. Until the perception of histology
> > as a second
> > > rate lab service is shaken, I fear that changes will be too
> > slow to avert
> > a
> > > crisis.
> > >
> > > Glen Dawson.
> > >
> > > -----Original Message-----
> > > From: Morken, Tim [mailto:tim9@cdc.gov]
> > > Sent: Monday, October 21, 2002 9:50 AM
> > > To: histonet@pathology.swmed.edu
> > > Subject: RE: The future of Histotechs
> > >
> > >
> > > Although I'm sure a lot of histotechs will retire in the next
> > 10-15 years,
> > I
> > > don't believe it will be in the 50 - 70 percent range. One
> > reason is that
> > as
> > > the shortage becomes worse, the pay goes up and labs will
> > accomadate older
> > > techs with incentives to keep them working longer - even as part
timers.
> > > I've already seen ads for partimers with full benefits. And per
> > diem work
> > > may beome common place. So, more realistically it may be more in the
30
> > > percent range, which is still bad!
> > >
> > > One bit of practical experience with this, from another field. My
mother
> > is
> > > a retired teacher who has been working about 75 percent of the
> > time since
> > > she retired. The benefit to her is she gets to pick her assignment, is
> > given
> > > full benefits and doesn't worry about all the extra stuff
> > teachers have to
> > > do these days. i thing something similar will happen with histotechs.
> > >
> > > Tim Morken
> > > Atlanta
> > >
> > > -----Original Message-----
> > > From: kevin williams [mailto:akwilliams75@hotmail.com]
> > > Sent: Sunday, October 20, 2002 9:48 AM
> > > To: histonet@pathology.swmed.edu
> > > Subject: The future of Histotechs
> > >
> > >
> > >
> > > At a one of the meetings at the ASCP in California there was an
> > interesting
> > > observation. I understand that in the next 5- 10 years between 50-70%
of
> > > histologists are going to retire.
> > > Can anyone tell me if there is definative research and where to get my
> > hands
> > >
> > > on it.
> > > Thanks in advance
> > > A. Kevin Williams
> > >
> > >
> > >
> > >
> > > _________________________________________________________________
> > > Surf the Web without missing calls! Get MSN Broadband.
> > > http://resourcecenter.msn.com/access/plans/freeactivation.asp
> > >
> > >
> >
> >
> >
>
>
>
>
> ----------------------------------------------------------------------
>
> Date: 27 Oct 2002 17:19:24 -0500
> From: Tony Henwood
> Subject: RE: CAM 5.2
>
>
> Alternatively
> Novocastra's NCL-5DE is reported to react equivalently. We use in place of
> Cam5.2 for our low molecular weight cytokeratins
>
> Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
> Laboratory Manager
> The Children's Hospital at Westmead,
> Locked Bag 4001, Westmead, 2145, AUSTRALIA.
> Tel: (02) 9845 3306
> Fax: (02) 9845 3318
>
> http://www.histosearch.com/homepages/TonyHenwood/default.html
> http://us.geocities.com/tonyhenwoodau/index.html
>
>
>
> - -----Original Message-----
> From: Dawson, Glen [mailto:GDawson@Milw.Dynacare.com]
> Sent: Saturday, 26 October 2002 1:52
> To: Edwards, R.E.; histonet@pathology.swmed.edu
> Subject: RE: CAM 5.2
>
>
> Richard,
>
> Becton Dickinson
>
> Cat. #349205.
>
> Proteinase K retrieval 4 min.
> LSAB2 Detection (DAKO)
> Dilution: 1:50 for 30 min.
>
> Works very well.
>
>
> Good Luck,
>
> Glen Dawson BS, HT & QIHC (ASCP)
> Lead IHC Technologist
> Milwaukee, WI
>
>
>
> - -----Original Message-----
> From: Edwards, R.E. [mailto:ree3@leicester.ac.uk]
> Sent: Friday, October 25, 2002 9:33 AM
> To: histonet@pathology.swmed.edu
> Subject: CAM 5.2
>
>
>
> Wanted a supplier of CAM5.2....................thanks
> Richard
Edwards
> MRC
> TOXICOLOGY UNIT......U.K.............
>
>
> **********************************************************************
> This email and any files transmitted with it are confidential and
> intended solely for the use of the individual or entity to whom they
> are addressed. If you are not the intended recipient, please
> delete it and notify the sender.
>
> Views expressed in this message and any attachments are those
> of the individual sender, and are not necessarily the views of the
> Childrens Hospital at Westmead
>
> This footnote also confirms that this email message has been
> virus scanned and although no computer viruses were detected,
> the Childrens Hospital at Westmead accepts no liability for any
> consequential damage resulting from email containing computer
> viruses.
> **********************************************************************
>
>
>
>
> ******************* NOTE *******************
> There may be important message content
> contained in the following MIME Information.
> ********************************************
>
>
> - ------------------ MIME Information follows ------------------
>
> This message is in MIME format. Since your mail reader does not understand
> this format, some or all of this message may not be legible.
>
> - --Boundary_(ID_nRoSSAYuxkTEWVRcShj29Q)
> Content-type: text/plain; charset=iso-8859-1
> Content-transfer-encoding: 7BIT
>
> <<<<<< See above "Message Body" >>>>>>
>
> - --Boundary_(ID_nRoSSAYuxkTEWVRcShj29Q)
> Content-type: text/html; charset=iso-8859-1
> Content-transfer-encoding: 7BIT
>
>
>
>
>
>
> RE: CAM 5.2
>
>
>
> Alternatively
>
Novocastra's NCL-5DE is reported to react equivalently.
We
> use in place of Cam5.2 for our low molecular weight
cytokeratins
>
> Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
>
Laboratory Manager
>
The Children's Hospital at Westmead,
>
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
>
Tel: (02) 9845 3306
>
Fax: (02) 9845 3318
>
>
> HREF="http://www.histosearch.com/homepages/TonyHenwood/default.html"
>
TARGET="_blank">http://www.histosearch.com/homepages/TonyHenwood/default.htm
l
>
TARGET="_blank">http://us.geocities.com/tonyhenwoodau/index.html
>
>
>
>
> -----Original Message-----
>
From: Dawson, Glen [
HREF="mailto:GDawson@Milw.Dynacare.com">mailto:GDawson@Milw.Dynacare.com
]
>
Sent: Saturday, 26 October 2002 1:52
>
To: Edwards, R.E.; histonet@pathology.swmed.edu
>
Subject: RE: CAM 5.2
>
>
>
> Richard,
>
>
> Becton Dickinson
>
>
> Cat. #349205.
>
>
> Proteinase K retrieval 4 min.
>
LSAB2 Detection (DAKO)
>
Dilution: 1:50 for 30 min.
>
>
> Works very well.
>
>
>
> Good Luck,
>
>
> Glen Dawson BS, HT & QIHC (ASCP)
>
Lead IHC Technologist
>
Milwaukee, WI
>
>
>
>
> -----Original Message-----
>
From: Edwards, R.E. [ HREF="mailto:ree3@leicester.ac.uk">mailto:ree3@leicester.ac.uk]
>
Sent: Friday, October 25, 2002 9:33 AM
>
To: histonet@pathology.swmed.edu
>
Subject: CAM 5.2
>
>
>
>
> Wanted a supplier of
> CAM5.2....................thanks
>
SIZE=2> &nb
sp; &
nbsp;
; &nb
sp;
> Richard Edwards
>
SIZE=2> &nb
sp; &
nbsp;
; &nb
sp; &
nbsp;
> MRC
>
TOXICOLOGY UNIT......U.K.............
>
>
>
>
> **********************************************************************
> This email and any files transmitted with it are confidential and
> intended solely for the use of the individual or entity to whom they
> are addressed. If you are not the intended recipient, please
> delete it and notify the sender.
>
> Views expressed in this message and any attachments are those
> of the individual sender, and are not necessarily the views of the
> Childrens Hospital at Westmead
>
> This footnote also confirms that this email message has been
> virus scanned and although no computer viruses were detected,
> the Childrens Hospital at Westmead accepts no liability for any
> consequential damage resulting from email containing computer
> viruses.
> **********************************************************************
>
<< Previous Message | Next Message >>