? Crystal Mount artefact

From:Anissa Choi



We run our immuno slides with AEC on Ventana immunostainer. After
staining, we place them in 1% Dawn detergent for 5 min to remove the liquid 
coverslip, followed by thorough washing in water. Slides are then covered 
with Crystal Mount and dry in oven for 20 to 30 min.
From time to time, we notice there are some tiny, black, refractile
round granules on top of the tissue section. Sometimes, it is so bad
that every part of the section is covered with this artefact granules,
which I think may be related to the Crystal Mount. I am seeking help  to 
solve this problem. Thanks in advance for your input.


Anissa Choi
Anatomical Pathology
Burnaby Hospital
Burnaby, B.C.
Canada




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