Bone sampling

From:Nick Bullough

Can anyone help with a nice little problem I have?

I need to sample both cortical and cancellous bone for histology and RNA
extraction. I've seen a dowel cutter that can be attached to a drill to take
cores of bone that will be ideal for what is needed.

My problem is that the health & safety people are very nervous about
aerosols and other contamination (bone dust). My idea was to enclose the
cutting in a box or behind a shield as it will be almost impossible to do
this within a safety cabinet (and there is still the problem of spray from
the cutter.

How do other people deal with bone sampling? any good ideas very gratefully

Best regards


Nick Bullough BSc(Hons) AIBMS
Pharmagene plc
2 Orchard Road
Herts SG8 5HD

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