Aqueous Mounting

From:John Baker

Hello all,

I'm trying to do a stain on bone cryosections that requires aqueous 
mounting (i.e. with GelMount from Biomeda).  I've been having trouble 
finding a bone counterstain that is stable in water.  Eosin and all 
the "Greens" (Fast green, light green) fade soon after mounting.  Is 
anyone aware of a bone stain/marker that won't fade?  Thanks in 
advance for any help.

John A. Baker
The University of Michigan
Orthopaedic Research Laboratories
Histology Unit
400 North Ingalls, G160
Ann Arbor, MI 48109-0486
Main lab office phone:734-763-9674
Histology office:734-936-1635

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