ways to soften hardened tissues

From:Kristel Wautier

Hello everybody,
for a change: a question on FISH tissues...
We are looking for ways to soften fish tissues that have been hardened due to fixation and storage in alcohol. We already have at our disposal the protocol for Vin d'Alsace, which can be used for this purpose. However, as Vin d'Alsace comprises a number of toxic products, we would like to try a less "dangerous" alternative.
The aim of our attempt: we need to straighten fish to record body measurements and such. So we are working with entire organisms...
All suggestions are welcome.
Kristel Wautier
Ghent University
Department of Biology
K.-L. Ledeganckstraat 35
9000 Ghent
Tel.: +32 9 264 52 31
Fax: +32 9 264 53 44

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