mouse tissue processing

From:"Martin, Ronald"

Fellow techs,
How long of a processing schedule would you use for mouse skin, pancreas and
salivary glands? 8 hours? 9 hours? 15 hours? A post doc I am doing work for
requested  a 15 hour processing schedule  now the tissue (esp. pancreas) is
dry (not overly dry but dry enough) and is difficult to section. I think a
shorter processing schedule will make the tissue easier to section. Any
comments?

Thanks in advance,
Ron Martin



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