I'm trying to section Xenopus oocytes frozen in OCT with poor results. Lots 
of cracking and shattering. I'm figuring that temp plays a roll here and 
the little things are very "yolky".
Is there anybody out in histonetland who may have done some sectioning of 
frog oocytes or something similar? I actually found a Xenopus histo book 
but no suggestions for cryosectioning.
As is usually the case here, the labs bring the tissue already frozen in 
OCT. What can I suggest they do prior to or during freezing the make the 
sections cut better?
Does anybody know an optimal temp for cutting frog oocytes?
Thanks for any help.

Andi Grantham
.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
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