Subject: microglial protocol
Dear Janet,
We sometimes use the Griffonia simplicifolia isolectin-B4 (Sigma) on rat
and mouse tissues. On paraffin we used 1h steaming with citrate buffer pH
6.0. The Isolectin-B4 staining in our hands always has been very irregular.
Certainly the degree of fixation plays an important role in succeeding or
not. Unfortunately there are little alternatives for staining all
microglial cells. OX-42 does not work on paraffin and ED1 only stains the
activated microglial cells (I come from the lab who created the ED
monoclonals). OX-6 (MHC class II) works after HIER but again only stains
the activated microglia. For cat tissues I cannot help.
Good luck
Jan
-----------------------------------------------------
Jan Bauer, Ph.D
Div. of Neuroimmunology
Brain Research Institute
Spitalgasse 4
A-1090 Vienna
Austria
tel: +43-1-4277 62813
fax: +43-1-4277 9628
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