Re: problems with toluidine blue stain

Hello everybody,
we were wondering if anybody had some advise on the following phenomenon: we have been using the toluidine blue stain on Epon tissues for many years now, and lately some strange things have been happening.
For some obscure reason the coloration of the tissue seems to fade away lately upon covering the slides using Epon as mounting medium. And this happens within half an hour. As we haven't made any changes to our methodology, we have no idea what might be the reason for this sudden change in results.
If anybody has suggestions, just let us know!
Kristel Wautier
Ghent University
Department of Biology
K.-L. Ledeganckstraat 35
9000 Ghent
Tel.: +32 9 264 52 31
Fax: +32 9 264 53 44

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