Re: mouse tissue processing


STF, Streck Tissue Fixative is a zinc based formalin substitute, manufactured
and distributed by STRECK Laboratories in Omaha, NE.  1-800-843-1912.
We use it exclusively for tissue fixation.  I was skeptical at first, I love
my formalin - but I've come to like this stuff fairly well for our purposes,
anyway.  I do IHC on virtually everything, and really like the results I've
seen with STF.  I don't know about long term storage in this, tho - we fix for
24-48 hours with great IHC results on CD31. CD34, Ki67, TUNEL, Caspase3 - so
far so good.
Jackie O'

                    Cristiano Rumio                                                                               
                                            Subject:     Re: mouse tissue processing                              
                    10/08/2002 09:54                                                                              

At 09.25 08/10/2002 -0500, you wrote:

>Ron -
>I process strictly mouse tissue - my protocol is 45 min in each station, 2x
>STF, 50% ETOH, 70% ETOH, 2x95% ETOH, 2x100% ETOH, 2x xylene, and 2x paraffin
>for a total of 9 hours.  I could probably go shorter, but this seems to work
>well for all tissue types, even teensy adrenals.  If the tissue has been
>for 24-48 hours, I skip the STF (Strecks Tissue Fixative) and start off in
>Good Luck.
>Jackie O'

>Dear Ron

>I am interested in your protocol but I do not know what "Strecks Tissue
>Fixative" is. Can you help me?

Thank you

Cristiano Rumio

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