Re: mouse tissue processing
At 09.25 08/10/2002 -0500, you wrote:
>Ron -
>I process strictly mouse tissue - my protocol is 45 min in each station, 2x
>STF, 50% ETOH, 70% ETOH, 2x95% ETOH, 2x100% ETOH, 2x xylene, and 2x paraffin -
>for a total of 9 hours. I could probably go shorter, but this seems to work
>well for all tissue types, even teensy adrenals. If the tissue has been fixed
>for 24-48 hours, I skip the STF (Strecks Tissue Fixative) and start off in
>50%.
>Good Luck.
>Jackie O'
>Dear Ron
>I am interested in your protocol but I do not know what "Strecks Tissue
>Fixative" is. Can you help me?
Thank you
Cristiano Rumio
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