Re: mouse tissue processing
At 09.25 08/10/2002 -0500, you wrote:
Ron -
I process strictly mouse tissue - my protocol is 45 min in each station,
2x
STF, 50% ETOH, 70% ETOH, 2x95% ETOH, 2x100% ETOH, 2x xylene, and 2x
paraffin -
for a total of 9 hours. I could probably go shorter, but this seems
to work
well for all tissue types, even teensy adrenals. If the tissue has
been fixed
for 24-48 hours, I skip the STF (Strecks Tissue Fixative) and start off
in
50%.
Good Luck.
Jackie O'
Dear Ron
I am interested in your protocol
but I do not know what "Strecks Tissue Fixative" is. Can you
help me?
Thank you
Cristiano Rumio
Prof. Cristiano Rumio
Department of Human Anatomy
Via Mangiagalli 31
20133 Milan
Italy
Tel. +39-02503155401
Fax. +39-0250315387
e-mail cristiano.rumio@unimi.it
http://www.anatomia.unimi.it/
Corso di Micorscopia
digitale:
http://www.istitutotumori.mi.it/int/areaprofessionale/formazione/pdf/programma.pdf
II Symposium: Cellular Biology of Stratified Epithelia
http://www.anatomia.unimi.it/didattica/se.htm
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