Re: mouse tissue processing

From:Cristiano Rumio

At 09.25 08/10/2002 -0500, you wrote:

Ron -
I process strictly mouse tissue - my protocol is 45 min in each station, 2x
STF, 50% ETOH, 70% ETOH, 2x95% ETOH, 2x100% ETOH, 2x xylene, and 2x paraffin -
for a total of 9 hours.  I could probably go shorter, but this seems to work
well for all tissue types, even teensy adrenals.  If the tissue has been fixed
for 24-48 hours, I skip the STF (Strecks Tissue Fixative) and start off in
Good Luck.
Jackie O'

Dear Ron

I am interested in your protocol but I do not know what "Strecks Tissue Fixative" is. Can you help me?

Thank you

Cristiano Rumio

Prof. Cristiano Rumio
Department of Human Anatomy
Via Mangiagalli 31
20133 Milan
Tel. +39-02503155401
Fax. +39-0250315387

Corso di Micorscopia digitale:

II Symposium: Cellular Biology of Stratified Epithelia

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