Re: mouse tissue processing

From:Jackie.O'Connor@abbott.com


Ron -
I process strictly mouse tissue - my protocol is 45 min in each station, 2x
STF, 50% ETOH, 70% ETOH, 2x95% ETOH, 2x100% ETOH, 2x xylene, and 2x paraffin -
for a total of 9 hours.  I could probably go shorter, but this seems to work
well for all tissue types, even teensy adrenals.  If the tissue has been fixed
for 24-48 hours, I skip the STF (Strecks Tissue Fixative) and start off in
50%.
Good Luck.
Jackie O'

Jacqueline M. O'Connor HT(ASCP)
Abbott Laboratories
Global Pharmaceutical Research and Development
Discovery Chemotheraputics
100 Abbott Parkway
Dept R4N2 Bldg AP3
Abbott Park, IL  60064
ph 847.938.4919
Jackie.O'Connor@abbott.com


                                                                                                                    
                    "Martin, Ronald"                                                                                
                                                                            
                                              cc:                                                                   
                    10/08/2002 08:30          Subject:     mouse tissue processing                                  
                    AM                                                                                              
                                                                                                                    
                                                                                                                    




Fellow techs,
How long of a processing schedule would you use for mouse skin, pancreas and
salivary glands? 8 hours? 9 hours? 15 hours? A post doc I am doing work for
requested  a 15 hour processing schedule  now the tissue (esp. pancreas) is
dry (not overly dry but dry enough) and is difficult to section. I think a
shorter processing schedule will make the tissue easier to section. Any
comments?

Thanks in advance,
Ron Martin








<< Previous Message | Next Message >>