Re: microglial protocol

From:Geoff McAuliffe

    Isolectin B4 is not specific for microglia (it stains small, unmyelinated
fibers in the spinal cord and a few other things that escape my memory this late
in the day) so an antibody is in order. Rat microglia react with several
different antibodies, OX-42 and ED-1 to name two of the more common ones. I
don't know about microglia in the cat. Microglia and macrophages often share
markers so an antibody for cat macrophages might work. Perhaps a literature
search on PubMed is in order?

"Miller, Janet" wrote:

> Hi, I am looking for a microglial protocol.  I tried using Isolectin B4, but
> can not get it to work. Does anyone have any suggestions on how to "Tweak"
> the ISo B4 protocol, or have any other suggestions.  I am staining both rat
> and cat tissues.  Any help is greatly appreciated!
> Thanks.
> Janet

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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