Re: em technique

From:Geoff McAuliffe

Unless you have a specific reason to use glutaraldehyde cold, I would use it at room temperature. Microtubules come out much better at room temp. Why would you want to use it cold?

Geoff

sueetyler@netscape.net wrote:

> I was wondering if you could give me your opinions regarding the refrigeration or non refrigeration of gluteraldehyde for em fixation. Do you have any good references on the subject?
>
> Sue Tyler, HT (ASCP)
> Oxford Laboratory
> styler@dnr.state.md.us
>

--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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