Re: em technique

From:Geoff McAuliffe

Unless you have a specific reason to use glutaraldehyde cold, I would use it at room temperature. Microtubules come out much better at room temp. Why would you want to use it cold?

Geoff wrote:

> I was wondering if you could give me your opinions regarding the refrigeration or non refrigeration of gluteraldehyde for em fixation. Do you have any good references on the subject?
> Sue Tyler, HT (ASCP)
> Oxford Laboratory

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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