Ryan,
JB4 is glycolmethacrylate and yes you can do trap, alk.p, not sure about
xgal.  we use cold absolute methanol fixative and cold processing.
Patsy

Ryan Blair Rountree wrote:

> Does anyone know if TRAP staining, Alk Phos staining, or LacZ staining
> (with Xgal) can be done on methylmethacrylate embedded (JB4 kit) calcified
> bone tissue?  If so, how did you fix and process the tissue?  Thanks
> again!
>
> Ryan
>
> Ryan Rountree
> Department of Developmental Biology
> B301, Beckman center
> 279 Campus Drive
> Stanford, CA 94305
> (650) 725-7599
> Fax: (650) 725-7739