Re: Paragon stain source

From:Geoff McAuliffe

You can make your own Paragon stain:

Dissolve 0.365 g of Toluidine Blue in 50 ml of 30% ethanol
then add 0.135 g of Basic Fuchsine and stir until dissolved.

I have seen some variations call for the addition of 0.75 g of sodium tetraborate but
this is not in the original recipe.

I believe the original references are:
Martin et al., Am. J. Clin Path. 46:250-251, 1966.
Spurlock et al., Am. J. Clin. Path. 46:252-258, 1966.
That's right, the papers are back to back in the same journal.

Cathy Mayton wrote:

Fellow Histonetters, I am looking for a source for the Paragon stain.  I have looked in all my catalogs and came up empty handed. Thank you in advance,Cathy****************************************************
"Quality Histology with a Personal Touch"
GLP Compliant Laboratory Cathy A. Mayton
Project Director
Wasatch Histo Consultants, Inc.

Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029

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