Dissolve 0.365 g of Toluidine Blue in 50 ml of 30% ethanol
then add 0.135 g of Basic Fuchsine and stir until dissolved.

I have seen some variations call for the addition of 0.75 g of sodium tetraborate but
this is not in the original recipe.

I believe the original references are:
Martin et al., Am. J. Clin Path. 46:250-251, 1966.
Spurlock et al., Am. J. Clin. Path. 46:252-258, 1966.
That's right, the papers are back to back in the same journal.

Cathy Mayton wrote:

Fellow Histonetters, I am looking for a source for the Paragon stain.  I have looked in all my catalogs and came up empty handed. Thank you in advance,Cathy****************************************************
"Quality Histology with a Personal Touch"
GLP Compliant Laboratory Cathy A. Mayton
Project Director
Wasatch Histo Consultants, Inc.
www.wasatchhisto.com

Geoff
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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mcauliff@umdnj.edu
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