Hi Patti:

We use Novocastra's mAb (NCL-CD22-2) at 1:100 for 30 minutes following
a 96 degree C. waterbath pretreatment for 40 minutes.  DAKO's EnVision+
and DAB+ are used for detection.

Rich Cartun

>>> Patti Loykasek  10/11/02 01:52PM >>>
Is anyone out there using CD22 on formalin fixed, paraffin embedded
tissue
with success? If so, would you please share your clone name and
pretreatment
with me? Thanks.

Patti Loykasek
Phenopath Laboratories
Seattle, WA