Re: Beta galactosidase antibody

From:Melanie Black


I am also trying to get this antibody to work. I have the abcam 
antibody (b-gal-ab616), but I have not been able to get it to work.
If you receive any good advice that does not go to histonet, I would 
appreciate it if you could forward it on to me.

Many Thanks

>Dear all,
>I need a good anti-beta-galactosidase antibody to detect expression in
>the thymus of my knockout mouse.  I have tried a rabbit polyclonal from
>Cappel, but it seems to stick to everything regardless of washing and
>blocking etc.  Does anyone have any recommendations, even if it has
>worked on another mouse tissue besides the thymus?
>Kylie Webster
>Genetics and Bioinformatics Division
>The Walter and Eliza Hall Institute of Medical Research
>Victoria, Australia


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